Cellular and Molecular Biotechnology

Welcome to the homepage of the research group of Prof. Kristian M. Müller

Research Summary

The Cellular and Molecular Biotechnology group aims at engineering biomolecules and cells for the benefit of mankind. We use means of genetic engineering and protein engineering in combination with synthetic biology approaches to create and deploy biosensors, bioproduction, and bio-pharmaceuticals. These application driven goals are accompanied by basic research aspiring to unravel the principles of biological systems based on their chemical and physical properties. 

Currently we focus on three research areas: i) recombinant Adeno-associated viruses for gene based prodrug activation therapy of cancer, ii) site specific modification of antibodies for the generation of antibody-drug conjugates, and iii) development of selection and directed evolution technologies. 

rAAV overview

recombinant Adeno Associated Viruses (rAAV)

rAAV are virus-derived nano-sized protein carriers of DNA developed by nature to deliver genes to eukaryotic cells. We engineer these particles to specifically transduce tumor cells and deliver genes for enzymes that promote tumor suicide upon administration of a prodrug. 

antibody FGE overview

Antibody Drug Conjugates

Site specific chemical modification of proteins is of utmost interest for controlling pharmacological properties. In cooperation with biochemists and organic chemists, we analyze how the formyl glycine generating enzyme (FGE) can be utilized to enable the efficient modification of antibodies. Antibody-drug conjugates extend the therapeutic potential of antibodies for tumor therapy.

selection overview

Selection and Directed Evolution

Utilizing the power of evolution at the molecular level in complex settings enables us to discover unknown or unexpected solutions while at the same time attaining molecules with desired properties. Therefore we aim at developing novel selection and evolution techniques or adapt existing techniques to our scientific challenges. So far we worked with phage display, TAT selection and protein fragment complementation assays.

Further Activities

Besides the current core projects, the group invests in enabling technologies, maintains previous projects and invests in education. Enabling technologies are, for example, software tools such as the NExt DNA shuffling prediction or a protocol assistance suite and previous projects comprise DNA origami nano structuring. The group was the host of several iGEM teams (from 2007 to 2010 in Freiburg and 2011-2012 in Potsdam) and actively supports the ongoing iGEM initiatives at Bielefeld University.

group image 2021
The Cellular and Molecular Biotechnology group in summer 2021.

Kristian Müller
Room UHG E2-143
Universitätsstr. 25
33615 Bielefeld, Germany

Tel. +49-521-106-6323 (office)
Tel. +49-521-106-6318 (secretary)
Fax. +49-521-106-156318
e-mail kristian@syntbio.net