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Coiled Coil Prediction

[Our mission]     [Equipment]     [Funding]     [Contact Information]                


Our mission

Most biological processes are mediated by interactions between proteins. Nonetheless, the molecular basis mediating specific protein-protein interactions remains incompletely understood. Our lab is interested in understanding the factors that mediate interactions in coiled-coil proteins. Coiled coils, which are helical ropes of two or more strands, are one of the most abundant protein interaction motifs. Coiled coils play major roles in processes as varied as transcription, cell signaling, viral infection, force generation, cell division, and fertilization.

Our goal is to target coiled-coil domains of proteins relevant to biology or medicine with small helical peptide probes. Important targets include proteins involved in tumorigenesis, tumor proliferation and metastasis. We are combining rational design strategies with library technologies and efficient selection systems. Peptide probes recognizing and blocking specific targets will reveal underlying signaling processes and might become important diagnostics and therapeutics.

Equipment

Our group owns a Circular Dichroism (CD) Spectrophotometer (Jasco J-810), a High Performance Liquid Chromatography (HPLC) System (ÄKTA Purifier 10), an isothermal titration calorimeter (ITC) as well as a well-equipped cell culture lab. In addition we are using the following equipment (listed from gene to protein): gradient PCR cyclers, qPCR, fermentation equipment at 37°C and 25°C, an Äkta HPLC with four ports and three wavelengths detection for protein purification, a BIAcore 3000 for interaction analysis, a temperature controlled UV-VIS spectrophotometer for absorption spectra and enzyme assays, a fluorimeter for interaction studies and stability measurements, a cell culture room equipped with two microscopes for cell based assays, a flow cytometer, a high resolution imaging system for gel and blot analysis, and networked personal computers for protein and gene design as well as data analysis. In addition, we have access to a MALDI and an ESI mass spectrometer and a sequencing service.

Funding

Our group is part of the School of Life Sciences in the Freiburg Institute for Advanced Studies (FRIAS-LIFENET), the cluster of excellence "biological signaling studies" (bioss) and the newly founded Collaborative Research Center 850 "Control of cell motility in morphogenesis, cancer invasion and metastasis". We receive additional funding from the Emmy-Noether Program of the Deutsche Forschungsgemeinschaft (DFG), from the José Carreras Leukemia Foundation and by the German Academic Exchange Service (DAAD). Currently, our group is in the transition of moving from the University of Freiburg to the University of Potsdam.

        

 

        

 

 

 

Contact Information

The lab is is located in room 04.024 on the fourth floor of the Institute for Biology III in the Department of Biology of the University of Freiburg in the city of Freiburg in the state Baden Württemberg in Germany. The following maps may guide you to the lab: the institute (87 kB), university buildings in Freiburg (84 kB), interactive city map of Freiburg, public transport, Freiburg and vincinity (306 kB), Germany (382 kB). Our new address in Potsdam will be in the Institute of Biochemistry and Biology in the Department for Mathematics and Natural Sciences of the University of Potsdam.

 

E-mail

Katja Arndt @ Uni Freiburg

Katja Arndt @ Uni Potsdam

 

 

Telephone

0761-203-2748

FAX  

0761-203-2745

Postal address Freiburg

FRIAS-LIFENET

Department of Biology III

University of Freiburg

Schänzlestr. 1

79104 Freiburg, Germany

   
Postal address Potsdam

Molecular Biotechnology

Institute for Biochemistry and Biology

University of Potsdam

Campus Golm, House 25

Karl-Liebknecht Str. 24-25

14476 Potsdam-Golm, Germany

 

[FRIAS]  [bioss]  [Institute for Biology III]  [Department of Biology] [University of Freiburg] [City of Freiburg]

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Last modified: 20-Apr-2010